Optimization of Conditions for Immobilization of Petroleum Degrad-ing Bacteria by Sodium Alginate and Its Auxiliary Effect

Abstract

With the development of marine oil pollution control technology, bioremediation technology has attracted much attention because of its low cost, high efficiency and environmental friendly characteristics, while solid bacteria technology can improve the degradation efficiency of bioremediation petroleum hydrocarbon degrading bacteria . In this study, sodium alginate and CaCl2 are used to carry out a fixed ball-forming test on petroleum hydrocarbon degrading bacteria, and four factors and three levels of orthogonal test are used to optimize the condition parameters (such as, sodium alginate concentration, bacteria suspension addition amount, CaCl 2 concentration and crosslinking time). The results show that the degradation efficiency of petroleum hydrocarbon degrading bacteria can be effectively improved by adopting a solid bacteria method. When the concentration of sodium alginate is 3%, the addition amount of bacteria suspension is 75%, CaCl 2 concentration 3% ,the cross-linking time is 30h to achieve the optimal immobilization effect, and the absolute degradation rate of petroleum hydrocarbon degrading bacteria microsphere 7d is 48． " 7% is 28%。 "higher than directly adding bacteria agent.