Study of Regulation Mechanism of F-P Optical Microcavity Quality Factor on ATPase Activity
Abstract
In this paper, the regulation mechanism of F-P microcavity quality factor on ATPase catalytic efficiency was studied. A microcavity with Q value of 20-100 was prepared for gold/silver/platinum microcavity. It was found that the catalytic efficiency of gold-plated microcavity reached 19.0% at Q=60, the activity of silver microcavity increased by 17.3% at Q=80, and the catalytic efficiency of platinum microcavity reached 22.1% at Q=100 due to the surface plasmon resonance effect and continued to rise. The kinetic simulation showed that the enzyme activity under the optimal Q values of the three microcavities showed a nonlinear concentration dependent relationship, which proved the synergistic effect of light field localization and enzymatic reaction. The smallest Km value (0.32 mM) and the largest Vmax value (0.85 μmol/min) of the platinum fi lm microcavity were obtained by Milloway equation fitting, corresponding to the highest substrate affi nity and enzyme catalytic efficiency, respectively. The results show that the high-Q microcavity can promote the catalytic reaction through the dual mechanism of enhancing enzyme activity and improving the ability of enzyme to bind to the substrate, which provides theoretical guidance for the development of photo-controlled enzyme catalytic devices.
Keywords
Optical microcavity; Quality factor; ATPase; ATPase activity; Kinetic analysis; Photoenzyme catalysis
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DOI: http://dx.doi.org/10.18686/ahe.v8i11.14079
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